Excellent result at Particle Tracking Challenge

Tracking individual biological particles in time-resolved fluorescence microscopy image data plays a central role in the quantitative analysis of the behavior of these structures. Tracking enables determining the position of structures over time, and a number of approaches have been developed in the past years.

To objectively compare the performance of several tracking approaches, for the first time, a Particle Tracking Challenge contest was held at the IEEE International Symposium on Biomedical Imaging (ISBI) 2012 in Barcelona, Spain. The Challenge consisted in automatically tracking fluorescent particles in 2D and 3D microscopy image sequences of four different application scenarios: vesicles, virus particles, receptors, and microtubule tips. For each scenario, different object densities and image noise levels were investigated. 14 groups world-wide participated in the Challenge, and different measures were used to quantify and rank the tracking approaches.

The results of the Challenge have been recently published in an article in the Nature Methods journal. The probabilistic tracking approach of the BMCV group (W.J. Godinez, K. Rohr) achieved the most accurate results overall.

Publication:

N. Chenouard, I. Smal, F. de Chaumont, M. Maška, I.F. Sbalzarini, Y. Gong, J. Cardinale, C. Carthel, S. Coraluppi, M. Winter, A.R. Cohen, W.J. Godinez, K. Rohr, Y. Kalaidzidis, L. Liang, J. Duncan, H. Shen, Y. Xu, K.E.G. Magnusson, J. Jaldén, H.M. Blau, P. Paul-Gilloteaux, P. Roudot, C. Kervrann, F. Waharte, J.Y. Tinevez, S.L. Shorte, J. Willemse, K. Celler, G.P. van Wezel, H.W. Dan, Y.S. Tsai, C. Ortiz de Solórzano, J.C. Olivo-Marin, E. Meijering: Objective comparison of particle tracking methods. Nature Methods (March 2014), Volume 11, Issue 3, 281-289, doi: 10.1038/nmeth.2808

Excellent result at Particle Tracking Challenge

Publication:

Related Links: