We are interested in studying how viruses interact with cellular organelles and how they remodeled cells to replicate. Many enveloped viruses such as influenza, SARS-CoV-2 or Ebola must cross the cell membrane during entry and exit. To do so, viruses developed or hijacked different protein machinery, which is able to remodel, fuse or cut membranes in processes dependent on specialized lipids. We use and further develop cryo-electron microscopy (cryo-EM) techniques such as cryo-focused ion beam milling (cryo-FIB), in situ cryo-electron tomography (cryo-ET) and cryo-correlative light and electron microscopy (cryo-CLEM) in conjunction with other imaging methods as fluorescence microscopy and imaging mass spectrometry to solve the puzzle of viral-membrane interactions.