Spatial lipidomics and viral infection


SIMS allows non-invasive imaging of chemically unmodified lipids with high chemical specificity. However, SIMS has so far been only performed on chemically fixed and dehydrated samples (sample preparation procedures known to severely alter membrane structure). In collaboration with Tom Wirtz (Luxembourg Institute of Science and Technology), we will apply SIMS to vitreous cryo-lamellas of the cells prepared by focused-ion beam milling to provide a spatial map of lipids in cellular organelles at native conditions. The lipid map will be subsequently correlated to the membrane structures observed by cryo-ET. We will study lipids such as cholesterol, sphigolipids and phosphatidylinositols, which are crucial in host-pathogen interactions as well as in membrane trafficking.


Correlative cryo-SIMS/ET
Contact: E-Mail (Last update: 25/10/2017)