Advanced Biological Screening Facility
Microscopy
"ready for DAQ" assays → high-throughput microscopy
Screening microscopes are used for high-throughput / high-content data acquisition. The facility provides three Olympus IX81 (Scan^R / Cell^R) and two Leica TCS SP5 microscopes. The microscopes are equipped with standard as well as screening data acquisition software.
- Olympus IX81
High-throughput screening
Wide field microscopes
- 3x Olympus Biosystems IX81 'inverted'
Wide field microscopes enable very rapid scanning procedures as well as time series of biological assays. For high-throughput wide field screening the microscopes can be loaded with various well plate formats.
- Two setups are equipped with incubator chambers allowing temperature, humidity and CO2 regulation for long term in vivo measurements.
- One setup facilitates automated plate loading via a stacker/gripper system for up to 40 well plates.
- Leica TCS SP5
- Perkin Elmer Opera LX
High-content screening
Confocal laser scanning microscopes
- 2x Leica TCS SP5 DMI6000 'inverted'
Spinning disk confocal screening microscopes
- Perkin Elmer Opera LX 'spinning disk'
In order to increase the information content, optical sectioning is indespensable. Confocal setups are used for high-content confocal screening providing 3-dimensional data stacks as well as time series of biological assays. The two Leica systems are equipped with an incubator chamber allowing temperature, humidity and CO2 regulation for long term in vivo measurements.
- Andor Dragonfly 505
High-resolution imaging
Spinning disk confocal microscope
- Andor Dragonfly 505
With this microscope we're able to acquire images at high- or even super-resolution. Due to the easy switch between different imaging techniques from wide field to spinning disk and to super-resolkution imaging by SRRF, the optimal conditions for each sample can be selected. The system is equipped with an okulab an incubator chamber allowing temperature, humidity and CO2 regulation for long term in vivo measurements.
Microscopes
Wide field screening microscopes
Olympus 1 (room 312, wall) Olympus Biosystems IX81 'inverted'
- illumination: Mercury (Hg) + Xenon (Xe) lamp
- detection: wide field
- sensor: CCD
- channels: 6 (DAPI, CFP, GFP, Cy3, Cy5, DAPI/FITC/TxRed)
- software: Scan^R / xcellence (data acquisition and analysis); versions: 2.3.0.5 (Scan^R data acquisition) | 1.3.0.3 (Scan^R analysis)
- probe handling: Object slides, standardised MTPs, cell arrays and adapter for various plate formats
- environment control: none
- automated plate loading: stacker/gripper system (up to 40 well plates)
- data storage: access to BioQuant internal mass storage (Large Scale Data Facility - LSDF)
Olympus 2 (room 312, door) Olympus Biosystems IX81 'inverted'
- illumination: Mercury (Hg) + Xenon (Xe) lamp
- detection: wide field
- sensor: CCD
- channels: 6 (DAPI, CFP, GFP, Cy3, Cy5, DAPI/FITC/TxRed)
- Software: Scan^R / xcellence (data acquisition and analysis); versions: 2.3.0.5 (Scan^R data acquisition) | 1.3.0.3 (Scan^R analysis)
- probe handling: Object slides, standardised MTPs, cell arrays and adapter for various plate formats
- environment control: incubator chamber allowing temperature, humidity and CO2 regulation
- automated plate loading: none
- data storage: access to BioQuant internal mass storage (Large Scale Data Facility - LSDF)
Olympus 3 (room 222, S2 area) Olympus Biosystems IX81 'inverted'
- illumination: Mercury (Hg) + Xenon (Xe) lamp
- detection: wide field
- sensor: CCD
- channels: 5 (DAPI, YFP, Cy5, CFP, GFP)
- Software: Scan^R / xcellence (data acquisition and analysis); versions: 2.3.0.5 (Scan^R data acquisition) | 1.3.0.3 (Scan^R analysis)
- probe handling: Object slides, standardised MTPs, cell arrays and adapter for various plate formats
- environment control: incubator chamber allowing temperature, humidity and CO2 regulation
- automated plate loading: none
- data storage: access to BioQuant internal mass storage (Large Scale Data Facility - LSDF)
Confocal microscope
SP5 (room 222, S2 area) Leica TCS SP5 DMI6000 'inverted'
- illumination: monochromatic light covering UV and visible spectrum:
- Diode laser (50 mW @ 405 nm)
- Ar+ laser (100 mW @ 458, 476, 488, 496, 514 nm)
- HeNe laser (1-10 mW @ 543, 594, 633 nm)
- DPSS laser (10 mW @ 561 nm)
- detection: confocal (continuously variable pinhole)
- sensor: photomultiplier (PMT)
- channels: whole VIS via Acousto Optical Beam Splitter (AOBS) and prism spectral detector (max. 5 channels in parallel)
- scanner: switch between conventional mode (5 fps @ 512x512 resolution) and resonant mode (25 fps @ 512x512)
- Software: Leica LAS AF (data acquisition and analysis, version 1.8.2)
- probe handling: Object slides, standardised MTPs, cell arrays and adapter for various plate formats
- environment control: incubator chamber allowing temperature, humidity and CO2 regulation
- data storage: access to BioQuant internal mass storage (Large Scale Data Facility - LSDF)
Confocal screening microscope
SP5 (room 312) Experimental Leica TCS SP5 DMI6000 'inverted'
- illumination: monochromatic light covering UV and visible spectrum:
- Diode laser (50 mW @ 405 nm)
- Ar+ laser (100 mW @ 458, 476, 488, 496, 514 nm)
- HeNe laser (1-10 mW @ 543, 594, 633 nm)
- DPSS laser (10 mW @ 561 nm)
- detection: confocal (continuously variable pinhole)
- sensor: photomultiplier (PMT)
- channels: whole VIS via Acousto Optical Beam Splitter (AOBS) and prism spectral detector (max. 5 channels in parallel)
- scanner: switch between conventional mode (5 fps @ 512x512 resolution) and resonant mode (25 fps @ 512x512)
- Software: Leica LAS AF (data acquisition and analysis) & Matrix Screener for automated confocal screening
- probe handling: Object slides, standardised MTPs, cell arrays and adapter for various plate formats.
- environment control: incubator chamber allowing temperature, humidity and CO2 regulation
- data storage: access to BioQuant internal mass storage (Large Scale Data Facility - LSDF)
Spinning disk confocal screening microscopes
Opera LX (room 222, S2 area) Perkin Elmer Opera LX 'spinning disk'
- illumination: monochromatic light covering visible spectrum:
- Diode laser (75 mW @ 488 nm)
- Diode laser (40 mW @ 561 nm)
- Diode laser (40 mW @ 640 nm)
- detection: confocal point scanning (spinning disk)
- objective lenses: 20x (NA=0.7), 60x (NA=1.2). Water immersion, automated water supply
- sensor: CCD camera (1.3 MegaPixel, 12bit)
- channels: whole VIS via dichroit / triple notch filter
- Software: Acapella. Online image processing
- probe handling: Object slides, standardised MTPs, cell arrays and adapter for various plate formats.
- data storage: access to BioQuant internal mass storage (Large Scale Data Facility - LSDF)
High-resolution microscope
Dragonfly (room U32, Spatial Transcriptomics Lab) Andor Dragonfly 505
- illumination: monochromatic light covering visible spectrum:
- Diode laser (405 nm)
- Diode laser (488 nm)
- Diode laser (514 nm)- Diode laser (561 nm)
- Diode laser (640 nm)
- detection: confocal point scanning (spinning disk) or wide field
- objective lenses: air: 10x (NA=0.7), 20x (NA=0.7), oil immerion: 40x (NA=1.3), 60x (NA=1.4), 60x (NA=1.49 TIRF), silicon immersion: 100x (NA=1.35).
- sensor: EM-CCD camera (1.05 MegaPixel, 16bit), SRRF-Streram Mode for super-resolution.
- channels: 5 laser illumination channels+ excitation filters.
- Software: Fusion, Imaris.
- probe handling: Object slides, standardised MTPs, cell arrays and adapter for various plate formats.
- environmental control: Okolab chamber for direct CO2 and temperature control, humidity readout.
- data storage: access to BioQuant internal mass storage (Large Scale Data Facility - LSDF).