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High-Content Analysis of the Cell (HiCell)

Dr. Holger Erfle

We specialize in devising, refining, and implementing state-of-the-art technologies for comprehensive screening and analysis.

In collaboration with the team "Integrin trafficking, microenvironment, and ncRNAs," these methodologies undergo development, validation, and standardization; they seamlessly integrate into the repertoire of the RNAi Screening Facility, becoming accessible to users. Within the HiCell group, our efforts are directed towards innovating methodologies to manipulate cellular functions across genome, transcript, and protein levels, streamlining cellular analyses through techniques such as cell arrays, advancing correlative light microscopy by merging high-speed and super-resolution microscopy techniques, and applying these cutting-edge technologies to address specific inquiries concerning the regulation of integrin membrane trafficking in response to environmental cues.

Research Strategy

The main focus of the “HiCell” research group is the design, development and application of novel technologies for high-content screening and analysis. Once developed, tested and standardized, the technologies will be incorporated into the portfolio of the Advanced Biological Screening Facility (ABSF) and made available to users at Heidelberg University’s life science campus. In HiCell, we are successfully addressing the following directions: 

  1. Development and optimization of novel methodologies to interfere with cell function at the genome, transcript, and protein levels. 

  2. Miniaturization of cellular analysis, e.g., cell arrays. Special focus is on the development and testing of High-Density Cell Arrays (HD-CA) that allow parallel and genome-wide cellular analysis of 24,576 samples in one experiment, e.g., ways to transfect active proteins thereon. 

  3. Development of targeted light microscopy combining high-speed and high-content microscopy, e.g., super-resolution microscopy

 

 

 

Erfle b&w
Dr. Holger Erfle
  • +49 (0)6221 54-51273

Selected Publications

Automated 3D light-sheet screening with high spatiotemporal resolution reveals mitotic phenotypes

Björn Eismann, Teresa G Krieger, Jürgen Beneke, Ruben Bulkescher, Lukas Adam, Holger Erfle, Carl Herrmann, Roland Eils, Christian Conrad

J Cell Sci.2020 Jun 1;133(11):jcs245043.

High-Density Cell Arrays for Genome-Scale Phenotypic Screening

Vytaute Starkuviene, Stefan M Kallenberger, Nina Beil, Tautvydas Lisauskas, Bastian So-Song Schumacher, Ruben Bulkescher, Piotr Wajda, Manuel Gunkel, Jürgen Beneke, Holger Erfle

SLAS Discov.2019 Mar;24(3):274-283.

Solid-phase reverse transfection for intracellular delivery of functionally active proteins

Ruben Bulkescher, Vytaute Starkuviene, Holger Erfle

Genome Res. 2017 Oct;27(10):1752-1758.