Single-Molecule Super-Resolution Microscopy

Research Strategy

Super-resolution fluorescence microscopy has evolved into a powerful tool for optical structural cell biology. By providing a spatial resolution at the protein size scale, it is beginning to revolutionize our understanding of cell biology. We operate various modalities of single-molecule localization microscopy (dSTORM, PALM, DNA-PAINT), implement novel protein labeling strategies to achieve many-target visualization (e.g., DNA-based protein labeling), and integrate quantitative data analysis tools that report on the molecular composition of single protein complexes in cells. Using neural networks, we establish rapid, multi-color super-resolution imaging workflows. We apply these super-resolution imaging techniques to study the structural organization of protein assemblies in cells.