Recent preprints from the lab:
Zimmermann L, Zhao X, Makroczyova J, Wachsmuth-Melm M, Prasad V, Bartenschlager R, Chlanda P SARS-CoV-2 nsp3-4 suffice to form a pore shaping replication organelles, bioRxiv, 21 Oct 2022, doi:10.1101/2022.10.21.513196
Selected publications from the lab:
2023
Zimmermann L, Chlanda P. Cryo-electron tomography of viral infection - from applications to biosafety. Curr Opin Virol. 2023 Aug;61:101338. doi: 10.1016/j.coviro.2023.101338.
Winter SL, Golani G, Lolicato F, Vallbracht M, Thiyagarajah K, Ahmed SS, Lüchtenborg C, Fackler OT, Brügger B, Hoenen T, Nickel W, Schwarz US, Chlanda P, The Ebola virus VP40 matrix undergoes endosomal disassembly essential for membrane fusion, EMBO J. 42(11):e113578. doi: 10.15252/embj.2023113578.
Klein S, Golani G, Lolicato F, Beyer D, Herrmann A, Wachsmuth-Melm M, Reddmann N, Brecht R, Lahr C, Hosseinzadeh M, Kolovou A, Schorb M, Schwab Y, Brügger B, Nickel W, Schwarz US, Chlanda P, (2023) IFITM3 blocks viral entry by sorting lipids and stabilizing hemifusion, Cell Host&Microbe, S1931-3128(23)0019-9, doi: 10.1016/j.chom.2023.03.005
2021
Winter SL, Chlanda P, (2021) Dual-axis Volta phase plate cryo-electron tomography of Ebola virus-like particles reveals actin-VP40 interactions, J Struct Biol, 107742. doi: 10.1016/j.jsb.2021.107742
Klein S, Wimmer WH, Winter SL, Kolovou A, Laketa V, Chlanda P, (2021) Post-correlation on-lamella cryo-CLEM reveals the membrane architecture of lamellar bodies. Communications Biology, 2021 Jan 29;4(1):137. doi: 10.1038/s42003-020-01567-z.
Klein S, Wachsmuth-Melm M, Winter SL, Kolovou A, Chlanda P. (2021) Cryo-correlative light and electron microscopy workflow for cryo-focused ion beam milled adherent cells. Methods Mol Biol. Correlative Light and Electron Microscopy, IV Volume 162, Chapter 12
2020
Klein S, Cortese M, Winter SL, Wachsmuth-Melm M, Neufeldt CJ, Cerikan B, Stanifer ML, Boulant S, Bartenschlager R, Chlanda P. (2020) SARS-CoV-2 structure and replication characterized by in situ cryo-electron tomography. Nature Communications, 11, 5885 doi.org/10.1038/s41467-020-19619-7
Klein S, Müller TG, Khalid D, Sonntag-Buck V, Heuser AM, Glass B, Meurer M, Morales I, Schillak A, Freistaedter A, Ambiel I, Winter SL, Zimmermann L, Naumoska T, Bubeck F, Kirrmaier D, Ullrich S, Barreto Miranda I, Anders S, Grimm D, Schnitzler P, Knop M, Kräusslich HG, Dao Thi VL, Börner K, Chlanda P. (2020), SARS-CoV-2 RNA Extraction Using Magnetic Beads for Rapid Large-Scale Testing by RT-qPCR and RT-LAMP. Viruses. 12(8):863. doi: 10.3390/v12080863.
