Recent preprints from the lab:
Vallbracht M, Bodmer SB, Fischer K, Makroczyova J, Winter SL, Wendt L, Wachsmuth-Melm M, Hoenen T, Chlanda P, Nucleocapsid condensation drives Ebola viral factory maturation and dispersion, bioRxiv, 7 Nov 2023, doi: https://doi.org/10.1101/2023.11.06.565679
Selected publications from the lab:
2023
Winter SL, Chlanda P, (2023) The Art of Viral Membrane Fusion and Penetration, Subcell Biochem, Book: Virus Infected cells. doi: 10.1007/978-3-031-40086-5_4.
Zimmermann L, Zhao X, Makroczyova J, Wachsmuth-Melm W, Prasad V, Hensel Z, Bartenschlager R, Chlanda P. (2023) SARS-CoV-2 nsp3 and nsp4 are minimal constituents of a pore spanning replication organelle. Nature Communications,14(1):7894. doi: 10.1038/s41467-023-43666-5
Zimmermann L, Chlanda P. Cryo-electron tomography of viral infection - from applications to biosafety. Curr Opin Virol. 2023 Aug;61:101338. doi: 10.1016/j.coviro.2023.101338.
Winter SL, Golani G, Lolicato F, Vallbracht M, Thiyagarajah K, Ahmed SS, Lüchtenborg C, Fackler OT, Brügger B, Hoenen T, Nickel W, Schwarz US, Chlanda P, (2023) The Ebola virus VP40 matrix undergoes endosomal disassembly essential for membrane fusion, EMBO J. 42(11):e113578. doi: 10.15252/embj.2023113578.
Klein S, Golani G, Lolicato F, Beyer D, Herrmann A, Wachsmuth-Melm M, Reddmann N, Brecht R, Lahr C, Hosseinzadeh M, Kolovou A, Schorb M, Schwab Y, Brügger B, Nickel W, Schwarz US, Chlanda P, (2023) IFITM3 blocks viral entry by sorting lipids and stabilizing hemifusion, Cell Host&Microbe, S1931-3128(23)0019-9, doi: 10.1016/j.chom.2023.03.005
2021
Winter SL, Chlanda P, (2021) Dual-axis Volta phase plate cryo-electron tomography of Ebola virus-like particles reveals actin-VP40 interactions, J Struct Biol, 107742. doi: 10.1016/j.jsb.2021.107742
Klein S, Wimmer WH, Winter SL, Kolovou A, Laketa V, Chlanda P, (2021) Post-correlation on-lamella cryo-CLEM reveals the membrane architecture of lamellar bodies. Communications Biology, 2021 Jan 29;4(1):137. doi: 10.1038/s42003-020-01567-z.
Klein S, Wachsmuth-Melm M, Winter SL, Kolovou A, Chlanda P. (2021) Cryo-correlative light and electron microscopy workflow for cryo-focused ion beam milled adherent cells. Methods Mol Biol. Correlative Light and Electron Microscopy, IV Volume 162, Chapter 12
2020
Klein S, Cortese M, Winter SL, Wachsmuth-Melm M, Neufeldt CJ, Cerikan B, Stanifer ML, Boulant S, Bartenschlager R, Chlanda P. (2020) SARS-CoV-2 structure and replication characterized by in situ cryo-electron tomography. Nature Communications, 11, 5885 doi.org/10.1038/s41467-020-19619-7
Klein S, Müller TG, Khalid D, Sonntag-Buck V, Heuser AM, Glass B, Meurer M, Morales I, Schillak A, Freistaedter A, Ambiel I, Winter SL, Zimmermann L, Naumoska T, Bubeck F, Kirrmaier D, Ullrich S, Barreto Miranda I, Anders S, Grimm D, Schnitzler P, Knop M, Kräusslich HG, Dao Thi VL, Börner K, Chlanda P. (2020), SARS-CoV-2 RNA Extraction Using Magnetic Beads for Rapid Large-Scale Testing by RT-qPCR and RT-LAMP. Viruses. 12(8):863. doi: 10.3390/v12080863.
