University of Heidelberg
BIOQUANT

ViroQuant-CellNetworks RNAi Screening Facility

Publications

 

  • Husedzinovic A, Neumann B, Reymann J, Draeger-Meurer S, Chari A, Erfle H, Fischer U and Gruss O (2015), The catalytically inactive tyrosine phosphatase HD-PTP/PTPN23 is a novel regulator of SMN complex localization, Mol Biol Cell, 26(2):161-171.
  • Poenisch M, Metz P, Blankenburg H, Ruggieri A, Lee JY, Rupp D, Rebhan I, Diederich K, Kaderali L, Domingues FS, Albrecht M, Lohmann V, Erfle H and Bartenschlager R (2015), Identification of HNRNPK as Regulator of Hepatitis C Virus Particle Production, PLoS Pathog., 8;11(1).
  • Gunkel M, Beil N, Beneke J, Reymann J and Erfle H (2015), Fluorescence microscopy-based RNA interference screening, Methods Mol Biol., 1251:59-66.


  • Vinnikov I, Hajdukiewicz K, Reymann J, Beneke J, Czajkowski R, Roth L, Novak M, Roller A, Dörner N, Starkuviene V, Theis F, Erfle H, Schütz G, Grinevich V and Konopka W (2014), Hypothalamic miR-103 protects from hyperphagic obesity in mice, J. Neurosci, 34(32):10659-10674.
  • Gunkel M, Flottmann B, Heilemann M, Reymann J and Erfle H (2014), Integrated and correlative high-throughput and superresolution
    microscopy, HistoChem Cell Biol, 141(6):597-603.
  • Zacherl S, La Venuta G, Müller HM, Wegehingel S, Dimou E, Sehr P, Lewis JD, Erfle H, Pepperkok R and Nickel W (2014), A Direct Role for ATP1A1 in Unconventional Secretion of Fibroblast Growth Factor 2, J Biol Chem., pii: jbc.M114.590067.
  • Marzenell P, Hagen H, Blechinger J, Erfle H and Mokhir A (2014), Terminally modified, short phosphorothioate oligonucleotides as inhibitors of gene expression in cells, Bioorg Med Chem Lett., 24(19):4694-8.
  • Eskova A, Knapp B, Matelska D, Reusing S, Arjonen A, Lisauskas T, Pepperkok R, Russell R, Eils R, Ivaska J, Kaderali L, Erfle H and Starkuviene V (2014), An RNAi screen identifies KIF15 as a novel regulator of the endocytic trafficking of integrin, J Cell Sci., 127(Pt 11):2433-47.


  • Blechinger J, Pieper H, Marzenell P, Kovbasyuk L, Serva A, Starkuviene V, Erfle H and Mokhir A (2013), Short, terminally modified 2'-OMe RNAs as inhibitors of microRNA, Chem Commun (Camb), 49(67):7397-9.
  • Flottmann B, Gunkel M, Lisauskas T, Heilemann M, Reymann J and Erfle H (2013), Correlative light microscopy for high-content screening, BioTechniques, 55(5):243-252.
  • Kuhns S, Schmidt K, Reymann J, Gilbert D, Neuner A, Hub B, Carvalho R, Wiedemann P, Zentgraf H, Erfle H, Klingmüller U, Boutros M and Pereira G (2013), 2. The microtubule affinity regulating kinase MARK4 promotes axoneme extension during early ciliogenesis, J Cell Biol, 200(4):505-22.
  • Börner K, Niopek D, Cotugno G, Kaldenbach M, Pankert T, Willemsen J, Zhang X, Schürmann N, Mockenhaupt S, Serva A, Hiet MS, Wiedtke E, Castoldi M, Starkuviene V, Erfle H, Gilbert DF, Bartenschlager R, Boutros M, Binder M, Streetz K, Kräusslich HG and Grimm D (2013), Robust RNAi enhancement via human Argonaute-2 overexpression from plasmids, viral vectors and cell lines, Nucleic Acids Res., 41(21).
  • Serva A, Knapp B, Tsai YT, Claas C, Lisauskas T, Matula P, Harder N, Kaderali L, Rohr K, Erfle H, Eils R, Braga V and Starkuviene V (2012), miR-17-5p Regulates Endocytic Trafficking through Targeting TBC1D2/Armus, PLoS One7(12):e52555.
  • Maiuri P, Terriac E, Paul-Gilloteaux P, Vignaud T, McNally K, Onuffer J, Thorn K, Nguyen PA, Georgoulia N, Soong D, Jayo A, Beil N, Beneke J, Lim JC, Sim CP, Chu YS; WCR participants, Jiménez-Dalmaroni A, Joanny JF, Thiery JP, Erfle H, Parsons M, Mitchison TJ, Lim WA, Lennon-Duménil AM, Piel M and Théry M (2012), The first World Cell Race, Curr Biol, 22(17):R673-5.
  • Simpson JC, Joggerst B, Laketa V, Verissimo F, Cetin C, Erfle H, Bexiga MG, Singan VR, Hériché JK, Neumann B, Mateos A, Blake J, Bechtel S, Benes V, Wiemann S, Ellenberg J and Pepperkok R (2012), Genome-wide RNAi screening identifies human proteins with a regulatory function in the early secretory pathway, Nat Cell Biol, 14(7):764-74.
  • Osterwald S, Wörz S, Reymann J, Sieckmann F, Rohr K, Erfle H and Rippe K (2012), A three-dimensional colocalization RNA interference screening platform to elucidate the alternative lengthening of telomeres pathway, Biotechnol J, 7:103-116.


  • Knapp B, Rebhan I, Kumar A, Matula P, Kiani NA, Binder M, Erfle H, Rohr K, Eils R, Bartenschlager R and Kaderali L (2011), Normalizing for individual cell population context in the analysis of high-content cellular screens, BMC Bioinformatics, 12:485.
  • Erfle H, Eskova A, Reymann J and Starkuviene V (2011), Microscopy-Based Integrin Endocytosis Assay, Methods in Mol Bio, doi: 10.1002/biot.201000474.
  • Lisauskas T, Matula P, Claas C, Reusing S, Wiemann S, Erfle H, Lehmann L, Fischer P, Eils R, Rohr K, Storrie B and Starkuviene V (2011), Live cell assays to identify regulators of ER to Golgi trafficking, Traffic, doi: 10.1111/j.1600-0854.2011.01318.x. Epub 2012 Jan 3.
  • Ritzerfeld J, Remmele S, Wang T, Temmerman K, Brügger B, Wegehingel S, Tournaviti S, Strating JR, Wieland FT, Neumann B, Ellenberg J, Lawerenz C, Hesser J, Erfle H, Pepperkok R and Nickel W (2011), Phenotypic profiling of the human genome reveals gene products involved in plasma membrane targeting of Src kinases, Genome Res, 21(11):1955-68.
  • Reiss S, Rebhan I, Backes P, Romero-Brey I, Erfle H, Matula P, Kaderali L, Poenisch M, Blankenburg H, Hiet MS, Longerich T, Diehl S, Ramirez F, Balla T, Rohr K, Kaul A, Bühler S, Pepperkok R, Lengauer T, Albrecht M, Eils R, Schirmacher P, Lohmann V and Bartenschlager R (2011), Recruitment and activation of a lipid kinase by hepatitis C virus NS5A is essential for integrity of the membranous replication compartment, Cell Host Microbe, 9(1):32-45.

  • Erfle H, Eskova A, Reymann J, Starkuviene V (2011), Cell arrays and high-content screening, Methods Mol Biol, 785:277-87.
  • Erfle H, Lisauskas T, Claas C, Reymann J and Starkuviene V (2011), Cell arrays for the measurement of organelle dynamics in living cells, Methods Mol Biol, 706:73-81.


  • Neumann B, Walter T, Heriche JK, Bulkescher J, Erfle H, Conrad C, Rogers P, Poser I, Held M, Liebel U, Cetin C, Sieckmann F, Pau G, Kabbe R, Wünsche A, Satagopan V, Schmitz MHA, Chapuis C, Gerlich GW, Schneider R, Eils R, Huber W, Peters JM, Hyman AA, Durbin R, Pepperkok R, and Ellenberg J (2010), Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes, Nature464(7289): 684-5.
  • Ebert AD, Ebert AD, Laußmann M, Wegehingel S, Kaderali L, Erfle H, Reichert J, Lechner J, Beer HD, Pepperkok R and Nickel W (2010), Tec-Kinase-mediated Phosphorylation of Fibroblast Growth Factor 2 is Essential for Unconventional Secretion, Traffic, 2010 Mar 10.
  • Börner K, Hermle J, Sommer C, Brown, N, Knapp B, Glass B, Kunkel J, Torralba G, Reymann J, Beil N, Beneke J, Pepperkok R, Schneider R, Ludwig T, Hausmann M, Hamprecht F, Erfle H, Kaderali L, Kräusslich HG and Lehmann M (2010), From experimental setup to bioinformatics: an RNAi screening platform to identify host factors involved in HIV-1 replication, Biotechnol J, 5(1):39-49.


  • Reymann J, Beil N, Beneke J, Kaletta PP, Burkert K and Erfle H (2009), Next-generation 9216-microwell cell arrays for high-content screening microscopy, BioTechniques, 47(4):877-878.
  • Reymann J, Lisauskas T, Erfle H and Starkuviene V (2009), High throughput and high content screening microscopy: A microscopy based guideline, European Pharm. Rev. 4:28-32.
  • Konrad A, Wies E, Thurau M, Marquardt G, Naschberger E, Hentschel S, Jochmann R, Schulz TF, Erfle H, Brors B, Lausen B, Neipel F and Stürzl M (2009), A systems biology approach to identify the combination effects of human herpesvirus 8 genes on NF-kappaB activation, J Virol. 83(6):2563-2574.


  • Matula P, Kumar A, Wörz I, Erfle H, Bartenschlager R, Eils R and Rohr K (2008), Single-cell-based image analysis of high-throughput screens using cell arrays for quantification of viral infection, Cytometry: Part A 75A(4):309-318.
  • Lemmer P, Gunkel M, Baddeley D, Kaufmann R, Urich A, Weiland Y, Reymann J, Müller P, Hausmann M and Cremer C (2008), SPDM: light microscopy with single-molecule resolution at the nanoscale, Appl Phys B 93:1-12
  • Starkuviene V, Sudziuviene U and Erfle H (2008), microRNAs for high throughput functional analysis, European Pharm. Rev. 3:10-17.
  • Reymann J, Baddeley D, Gunkel M, Lemmer P, Stadter W, Jegou T, Rippe K, Cremer C and Birk U (2008), High-precision structural analysis of subnuclear complexes in fixed and live cells via spatially modulated illumination (SMI) microscopy, Chrom. Res. 16:367-382
  • Erfle H, Neumann B, Rogers P, BulkescherJ, Ellenberg J and Pepperkok R (2008), Workflow for multiplexing siRNA assays by solid phase reverse transfection in multi-well plates, J. Biomol. Screen. 13(7):575-580.
  • Starkuviene V, Seitz A, Erfle H and Pepperkok R (2008), Measuring secretory  membrane traffic: a quantitative fluorescence microscopy approach, Meth. Mol. Biol. 457:193-201.
  • Tegha-Dunghu J, Neumann B, Reber S, Krause R, Erfle H, Walter T, Held M, Rogers P, Hupfeld K, Ruppert T, Ellenberg J and Gruss OJ (2008), Eml3 is a Nuclear Microtubule-binding protein required for proper Alignment of Chromosomes in Metaphase, J. Cell Sci. 121(Pt 10):1718-1726.
  • Stürzl M, Konrad A, Sander G, Wies E, Neipel F, Naschberger E, Reipschläger S, Gonin-Laurent N, Horch RE, Kneser U, Hohenberger W, Erfle H and Thurau M (2008), High Throughput Screening of Gene Functions in Mammalian Cells Using Reversely Transfected Cell Arrays: Review And Protocol, CCHTS 11:159-172.


  • Starkuviene V, Pepperkok R and Erfle H (2007), Transfected cell  microarrays: an efficient tool for high-throughput functional analysis,  Expert Rev. Proteomics 4(4):479-489.
  • Erfle H, Neumann B, Liebel U, Rogers P, Held M, WalterT, Ellenberg J and Pepperkok R (2007), Reverse transfection on cell arrays for high content screening microscopy, Nature Prot. 2(2):392-399.
  • Simpson JC, Cetin C, Joggerst B, Liebel U, Erfle H, Ellenberg J, Pepperkok R (2007), An RNAi screening platform to identify secretion machinery in mammalian cells, Journal of Biotechnology 129:352-365.
  • Erfle H and Pepperkok R (2007), Production of siRNA and cDNA transfected cell arrays on noncoated chambered coverglass for high content screening microscopic in living cells, Methods in Molecular Biology 360:155 – 162.
  • Starkuviene V, Pepperkok R and Erfle H (2007), Transfected cell microarrays: an efficient tool for high-throughput functional analysis, Expert Rev Proteomics 4(4):479-89.
  • Erfle H, Starkuviene V und Eils R (2007), Knock-down auf dem Zellchip , BIOspektrum, Sonderheft zur Biotechnika :36-37.
  • Simpson JC, Cetin C, Erfle H and Pepperkok R (2007), Genome-wide RNAI screening for membrane traffic molecules, Cytometrie Part A 71A.


  • Neumann B*, Held M*, Liebel U*, Erfle H*, Rogers P, Pepperkok R  and Ellenberg J (2006) *authors contributed equally to this publication, High-throughput RNAi screening by time-lapse imaging of live human cells, Nature Methods 3(5):385-90. PubMed.
  • Erfle H (2006), Funktionelle Proteomik mittels Transfektionszellarrays, Bioforum 5:14-15.
  • Erfle H, Liebel U, Neumann B, Pepperkok R und Ellenberg J (2006), RNAi-Screening mittels Lebendzellchips und automatischer Fluoreszenzmikroskopie, BIOspektrum • Sonderausgabe • 11. Jahrgang :521.
  • Erfle H, Liebel U, Neumann B, Ellenberg J and Pepperkok R (2006), RNAi auf Lebendzellchips 22.000 Gene einzeln ausschalten, Analytika PRO :20-23.


  • Erfle H and Pepperkok R (2005), Arrays of transfected mammalian cells for high content screening microscopy, Methods Enzymol. 404:1-8. PubMed.


  • Starkuviene V, Liebel U, Simpson JC, Erfle H, Poustka A, Wiemann S and Pepperkok R (2004), High-content screening microscopy identifies novel proteins with a putative role in secretory membrane traffic, Genome Res 14(10A):1948-1956. PubMed.
  • Erfle H, Simpson JC, Bastiaens PI and Pepperkok R (2004), siRNA cell arrays for high-content screening microscopy, Biotechniques 37(3):454-458, 460, 462. PubMed.
  • Conrad C*, Erfle* H, Warnat P, Daigle N, Lorch T, Ellenberg J, Pepperkok R and & Eils R (2004) *authors contributed equally to this publication, Automatic identification of subcellular phenotypes on human cell arrays, Genome Res. 14(6):1130-1136. PubMed.


  • Liebel U, Starkuviene V, Erfle H, Simpson JC, Poustka A, Wiemann S and Pepperkok R (2003), A microscope-based screening platform for large-scale functional protein analysis in intact cells, FEBS Lett 554(3):394-398. PubMed.


  • Salanoubat M, Lemcke K, Rieger M, Ansorge W, Unseld M, Fartmann B, Valle G, Blöcker H, Perez-Alonso M, Obermaier B, Delseny M, Boutry M,     Grivell LA, Mache R, Puigdomenech P, De Simone V, Choisne N, Artiguenave F, Robert C, Brottier P, Wincker P, Cattolico L, Weissenbach J, Saurin W, Quetier F, Schafer M, Muller-Auer S, Gabel C, Fuchs M, Benes V, Wurmbach E, Drzonek H, Erfle H, Jordan N, Bangert S, Wiedelmann R, Kranz H, Voss H, Holland R, Brandt P, Nyakatura G, Vezzi A, D'Angelo M, Pallavicini A, Toppo S, Simionati B, Conrad A, Hornischer K, Kauer G, Lohnert TH, Nordsiek G, Reichelt J, Scharfe M, Schon O, Bargues M, Terol J, Climent J, Navarro P, Collado C, Perez-Perez A, Ottenwalder B, Duchemin D, Cooke R, Laudie M, Berger-Llauro C, Purnelle B, Masuy D, de Haan M, Maarse AC, Alcaraz JP, Cottet A, Casacuberta E, Monfort A, Argiriou A, Flores M, Liguori R, Vitale D, Mannhaupt G, Haase D, Schoof H, Rudd S, Zaccaria P, Mewes HW and Mayer KF (2000), Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana, Nature 408(6814):820-822.


  • Erfle H, Ventzki R, Voss H, Rechmann S, Benes V, Stegemann J, Ansorge W (1997), Simultaneous loading of 200 sample lanes for DNA sequencing on vertical and horizontal, standard and ultrathin gels, Nucleic Acids Res. 25:2229-2230.
  • Zheng L, Cornel AJ, Wang R, Erfle H, Voss H, Ansorge W, Kafatos FC and Collins FH (1997), Quantitative trait loci for refractoriness of Anopheles gambiae to Plasmodium cynomolgi B, Science 276:425-428.


  • Wiemann S, Rechmann S, Benes V, Voss H, Schwager C, Vlcek C, Stegemann J, Zimmermann J, Erfle H, Paces V, Ansorge W (1996), Sequencing and analysis of 51kb on the right arm of chromosome XV from Saccaromyces cerevisiae reveals 30 open reading frames, Yeast 12:281-288.


  • Voss H, Schwager C, Wiemann S, Zimmermann J, Stegemann J, Erfle H, Voie AM, Drzonek H, Ansorge WJ (1995), Efficient low redundancy large scale DNA sequencing at EMBL, Biotechnol. 41:121-129.


  • Zimmermann J, Voss H, Wiemann S, Erfle H, Rupp T, Hewitt NA, Schwager C, Stegemann J and Ansorge W (1994), Direct sequencing of PCR products using magnetic beads and Fluorescein-15-dATP, Methods in Molecular and Cellular Biology 4:29-32.
  • Dujon B, Alexandraki D, Andre B, Ansorge W, Baladron V, Ballesta JPG, Banrev A, Bolle PA, Bolotin-Fukuhura M, Bossier P, Bou G, Boyer J, Bultrago MJ, Cheret G, Colleaux L, Dalgnan-Fornier B, del Rey F, Dion C, Domdey H, Düsterhoft A, Düsterhus S, Entian K-D, Erfle H, Esteban PF, Feldmann H, Fernandes L, Fobo GM, Fritz C, Fukuhara H, Gabel C, Gaillon L, Carcia-Cantalejo JM, Garcia-Ramirez JJ, Gent ME, Ghazvini M, Goffeau A, Gonzales A, Grothues D, Guerreiro P, Hegemann J, Hewitt NA, Hilger F, Hollenberg CP, Horaitis O, Indge KJ, Jaquire A, James CM, Jauniaux JC, Jiminez A, Keuchel H, Kirchrath L, Kleine K, Kötter P, Legrain P, Liebl S, Louis EJ, Maria e Silva A, Marck C, Monnier C, Möstl D, Müller S, Obermeier B, Oliver SG, Pallier C, Pascolo S, Pfeiffer F, Philippsen P, Planat RJ, Pohl FM, Pohl TM, Pöhlmann R, Portetelle D, Purnelle B, Puzos V, Ramezani Rad M, Rasmussen SW, Remacha M, Revuelta JL, Richard G, Rieger M, Rodrigues-Pousada C, Rose M, Rupp T, Santos MA, Schwager C, Sensen C, Skala J, Soarez H, Sor F, Stegemann J, Tettelin H, Thierry M, Tzermia M, Urrestarazu LA, van Dyck L, van Vliet-Reedijk JC, Valens M, Vandenbol M, Vilela C, Vissers S, von Wettstein D, Voss H, Wiemann S, Xu G, Zimmermann J, Haasemann M, Becker I and Mewes HW (1994), Complete DNA Sequence of yeast XI, Nature 369:371-378.


  • Grothues D, Voss H, Stegemann J, Wiemann S, Sensen C, Zimmermann J, Schwager C, Erfle H, Rupp T, Ansorge W (1993), Separation of up to 1000 bases on a modified A.L.F. DNA sequencer, Nucleic Acids Res. 21:6042-6044.
  • Wiemann S, Voss H, Schwager C, Rupp T, Stegemann J, Zimmermann J, Grothues D, Sensen C, Erfle H, Hewitt N, Banrevi A, Ansorge W (1993), Sequencing and analysis of 51,6 kilobases on the left arm of chromosome XI from Saccaromyces cerevisiae reveals 23 open reading frames including the FAS1 gene, Yeast 9:1343-1348.
  • Zimmermann J, Voss H, Erfle H, Rupp T, Dietrich T, Hewitt NA, Schwager C, Stegemann J, Ansorge W (1993), Direct Sequencing of PCR Products using Magnetic Beads and Fluorescein-12-dUTP, Methods in Molecular and Cellular Biology 3:114-115.
  • Zimmermann J, Voss H, Wiemann S, Erfle H, Rupp T, Hewitt NA, Schwager C, Stegemann J and Ansorge W (1993), Cycle Sequencing protocol with Fluorescein-12-dCTP, Methods in Molecular and Cellular Biology 4:27-28.
  • Voss H, Wiemann S, Grothues D, Sensen C, Zimmermann J, Schwager C, Stegemann J, Erfle H, Rupp T and Ansorge W (1993), Automated low-redundancy large-scale sequencing by primer walking, Biotechniques 15:714-721.
  • Ansorge W, Zimmermann J, Erfle H, Hewitt N, Rupp T, Schwager C, Sproat B, Stegemann J and Voss H (1993), Sequencing reactions for A.L.F. (EMBL) automated DNA sequencer. European Molecular Laboratory, Methods Mol. Biol. 23:317-356.


  • Ansorge W, Voss H, Wiemann S, Schwager C, Sproat B, Zimmermann J, Stegemann J, Erfle H, Hewitt N and Rupp T (1992), High-throughput automated DNA sequencing facility with fluorescent labels at the European Molecular Biology Laboratory, Electrophoresis 13:616-619.
  • Zimmermann J, Dietrich T, Voss H, Erfle H, Schwager C, Stegemann J, Hewitt NA and Ansorge W (1992), Fully Automated Sanger Sequencing Protocol for Double Stranded DNA, Methods in Molecular and Cellular Biology 3:39-42.
  • Voss H, Stegemann J, Schwager C, Zimmermann J, Erfle H, Hewitt NA, Rupp T and Ansorge W (1992), High-Speed Automated DNA Fragment Analysis for Genome Mapping by Restriction Fingerprinting, Methods in Molecular and Cellular Biology 3:77-82.
  • Zimmermann J, Voss H, Wiemann S, Erfle H, Rupp T, Hewitt NA, Schwager C, Stegemann J and Ansorge W (1992), Cycle Sequencing Protocol with Fluorescein-12-dUTP for M13, Plasmid and Cosmid DNA, Methods in Molecular and Cellular Biology 3:114-115.


  • Stegemann J, Schwager C, Erfle H, Hewitt NA, Voss H, Zimmermann J, and Ansorge W (1991), High speed on-line DNA sequencing on ultrathin slab gels, Nucleic Acids Res. 19:675-676.


  • Ansorge W, Zimmermann J, Schwager C, Stegemann J, Erfle H and Voss H  (1990), One label, one tube, Sanger DNA sequencing in one and two lanes on a gel, Nucleic Acids Res. 18:3419-3420.
  • Edwards A, Voss H, Rice P, Civitello A, Stegemann J, Schwager C, Zimmermann J, Erfle H, Caskey CT and Ansorge W (1990), Automated DNA sequencing of the human HPRT locus, Genomics 6:593-608.
  • Voss H, Zimmermann J, Schwager C, Erfle H, Stegermann J, Stucky K and Ansorge W (1990), Automated fluorescent sequencing of cosmid DNA, Nucleic Acids Res. 18:1066.
  • Zimmermann J, Voss H, Schwager D, Stegemann J, Erfle H, Stucky K,     Kristensen T and Ansorge W (1990), A simplified protocol for fast plasmid DNA sequencing, Nucleic Acids Res. 18:1067.
  • Voss H, Zimmermann J, Schwager C, Erfle H, Stegemann J, Stucky K and Ansorge W (1990), Automated fluorescent sequencing of lambda DNA, Nucleic Acids Res. 18:5314.
  • Rosenthal A, Sproat B, Voss H, Stegemann J, Schwager C, Erfle H, Zimmermann J, Coutelle C and Ansorge W (1990), Automated sequencing of fluorescent label DNA by chemical degradation, DNA Seq. 1:63-71.


  • Zimmermann J, Voss H, Kristensen T, Schwager C, Stegemann J, Erfle H and Ansorge W (1989), Automated Preparation and Purification of M13 Templates for DNA Sequencing, Methods in Molecular and Cellular Biology 1:29-34.
  • Voss H, Schwager C, Kristensen T, Duthie S, Olsson A, Erfle H, Stegemann J, Zimmermann J and Ansorge W (1989), One-Step Reaction Protocol for Automated DNA Sequencing with T7 DNA Polymerase, Methods in Molcular and Cellular Biology 1:155-159.
  • Voss H, Schwager C, Wirkner U, Sproat B, Zimmermann J, Rosenthal A, Erfle H, Stegemann J and Ansorge W (1989), Direct genomic fluorescent on-line sequencing and analysis using in vitro amplification of DNA, Nucleic Acids Res. 17:2517-2527.
  • Ansorge W, Voss H, Wirkner U, Schwager C, Stegemann J, Pepperkok R, Zimmermann J and Erfle H (1989), Automated Sanger DNA sequencing with one label in less that four lanes on gel, J. Biochem. Biophys. Methods 20:47-52.
Contact: E-Mail (Last update: 12/02/2015)