Molecular Mechanisms of Intracellular Trafficking

Research Strategy

Technology: As part of a collaborative effort (Erfle group), we have developed quantitative cell-based assays and high-content imaging techniques that enable the identification and characterization of numerous novel trafficking regulators. We manipulate cellular functions across all major regulatory levels: at the DNA level via CRISPR-Cas9 gene editing, at the RNA level via RNA interference, and at the protein level via antibody-mediated loss-of-function. The combined use of these three experimental modalities enhances the output of phenotype-based screens and strengthens the validation of their results.

3D models: To achieve greater physiological relevance, we focus on investigating the principles of intracellular trafficking in 3D cell culture systems. In collaboration with data modeling groups (Kummer and Sahle group), we aim to develop predictive models of cargo diffusion, internalization, and endosomal release within these 3D environments. To this end, we study individual cells using high-resolution microscopy and single-cell analysis methods (Rippe group)

Biomedical models: Ultimately, we aim to characterize trafficking processes in disease-relevant contexts. In collaboration with Prof. Michael Keese, we are focusing on 2D and 3D cellular models of cardiovascular diseases. Our current model system involves vascular smooth muscle cells (VSMCs), which respond to numerous intra- and extracellular cues by transitioning between a healthy differentiated (contractile) and an unhealthy dedifferentiated (synthetic) state. We investigate how intracellular trafficking pathways contribute to the regulation of this phenotypic switch, with a focus on identifying specific trafficking events and molecular players that modulate this cellular transition.